PyTom: Cut particles from a volume
Here, you will learn how to cut particles from a volume instead of reconstruction from the projections
Using the Pytom Script
If you have a volume and want simply cut some subvolumes out of it given the particle list containing the coordinates, you can achieve that by:
NAME cutParticlesFromVol.py DESCRIPTION Extract candidate molecules from localization result. OPTIONS -v, --volume Localization job XML file. (Is optional: No; Requires arguments: Yes) -p, --particleFile . (Is optional: No; Requires arguments: Yes) -c, --cubeSize Cube size along each dimension. (Is optional: No; Requires arguments: Yes) --help Print this help. (Is optional: Yes; Requires arguments: No) AUTHORS Yuxiang Chen
So what you have to prepare is the partile list file containing the center coordinates of your particles. Please refer to automatic localization or manual localization if you do not know how to do this.
As a result, all the partiles with the size dim^3 will be cut out and written to the disk specified by the filenames in the particle list. In case some particles are not cut out because they are too close to the volume boundary, a new particle list containing all cut out particles will be written into the current working directory. Please use this new particle list for further steps.